专家人才库数据----中国科学院上海巴斯德研究所

Principal investigator2016

Name:	Jin ZHONG	Gender:
Education:
Academic degree:	PhD	Academic title:	Principal Investigator
Departments:	Viral hepatitis	Discipline:	Virology
Phone:	021-54923143	E-mail:	jzhong@sibs.ac.cn
Mailing Address:	320 Yueyang Road, Shanghai, China 200031

Curriculum vitae:
2016 CAS Distinguished Research Fellow Program 　　2015 2015 Emergences Award (French Embassy) 　　2014 CAS Excellent Graduate Supervisor Award 　　2013 China State Council Expert for Special Allowance Award 　　2012 CAS Excellent Teacher Award 2012 CAS Shanghai Branch Talents Excellence Nominee Award 　　2013-present Adjunct Professor, College of Life Sciences, Shanghai Tech University. 　　2007-present Professor, Principal Investigator, the Unit of Viral Hepatitis, Institut Pasteur of Shanghai, Chinese Academy of Sciences. 2003-2007 Postdoctoral fellow, The Scripps Research Institute, USA August, 2003 Ph.D., Microbiology, University of Texas at Austin, USA 　 July, 1997 M.S., Biochemistry and Molecular Biology, Peking University, China. 　 July, 1994 B.S., Biochemistry, Sichuan University, China.

Curriculum vitae:

　　2016 CAS Distinguished Research Fellow Program

　　2015 2015 Emergences Award (French Embassy)

　　2014 CAS Excellent Graduate Supervisor Award

　　2013 China State Council Expert for Special Allowance Award

　　2012 CAS Excellent Teacher Award

2012 CAS Shanghai Branch Talents Excellence Nominee Award

　　2013-present Adjunct Professor, College of Life Sciences, Shanghai Tech University.

　　2007-present Professor, Principal Investigator, the Unit of Viral Hepatitis, Institut Pasteur of Shanghai, Chinese Academy of Sciences.

2003-2007 Postdoctoral fellow, The Scripps Research Institute, USA

August, 2003 Ph.D., Microbiology, University of Texas at Austin, USA

　 July, 1997 M.S., Biochemistry and Molecular Biology, Peking University, China.

　 July, 1994 B.S., Biochemistry, Sichuan University, China.

Research direction:
Hepatitis C virus (HCV) causes chronic hepatitis, liver cirrhosis and liver cancer, and is a major worldwide human health problem. Introduction of highly effective direct-acting antiviral agents has greatly improved the therapeutic treatment of chronic hepatitis C in recent years, but new challenges remain such as viral drug-resistant mutations. Furthermore, lack of HCV vaccine makes it impossible to eradicate HCV globally. As a highly variable RNA virus, HCV renders a unique model to study virus-host interactions particularly for its association with host lipoproteins. Our main interest is to elucidate the molecular basis of virus-host interactions during HCV infection, which may contribute to the prevention and better treatment of HCV infection. On the other hand, we hope that our research will help address important basic scientific questions in the field of virology, immunology and cell biology. In the last several years, our research interests have been expanded to other medically important RNA viruses, such as Zika virus and Ebola virus.

Research direction:

Hepatitis C virus (HCV) causes chronic hepatitis, liver cirrhosis and liver cancer, and is a major worldwide human health problem. Introduction of highly effective direct-acting antiviral agents has greatly improved the therapeutic treatment of chronic hepatitis C in recent years, but new challenges remain such as viral drug-resistant mutations. Furthermore, lack of HCV vaccine makes it impossible to eradicate HCV globally. As a highly variable RNA virus, HCV renders a unique model to study virus-host interactions particularly for its association with host lipoproteins. Our main interest is to elucidate the molecular basis of virus-host interactions during HCV infection, which may contribute to the prevention and better treatment of HCV infection. On the other hand, we hope that our research will help address important basic scientific questions in the field of virology, immunology and cell biology. In the last several years, our research interests have been expanded to other medically important RNA viruses, such as Zika virus and Ebola virus.

Research progress:
1. ^#Tong, Y., Lavillette, D., Li, Q. and ^#Zhong, J. (2018) Role of hepatitis c virus envelope glycoprotein E1 in virus entry and assembly. Front. Immunol. 9:1411. doi: 10.3389/fimmu.2018.01411 (^#corresponding author). 2. Liang, Y., Cao, X., Ding, Q., Zhao, Y., He, Z., Zhong, J. (2018) Hepatitis C virus NS4B induces the degradation of TRIF to inhibit TLR3-mediated interferon signaling pathway. PLOS Pathogens. 14(5):e1007075 (The first two authors contributed equally). 3. Li, Q., Tong Y., Xu, Y., ^#Niu, J., ^#Zhong, J.* (2018) Genetic Analysis of Serum-derived Defective Hepatitis C Virus Genomes Revealed Novel Viral Cis-Elements for Virus Replication and Assembly. J. Virology, 92(7). pii: e02182-17 (^#corresponding author). 4. Wang, X., Yan, Y., Gan, T., Yang, X., Li, D., Zhou, D., Sun, Q., ^#Huang, Z., ^#Zhong, J. (2017) A trivalent HCV vaccine elicits broad and synergistic polyclonal antibody response in mice and rhesus monkey. Gut, doi: 10.1136/gutjnl-2017-314870. (The first two authors contributed equally;^#corresponding author). 5. Tao, W., Gan T., Guo, M., Xu, Y., Zhong, J.* (2017) Novel Stable Ebola Virus Minigenome Replicon Reveals Remarkable Stability of the Viral Genome. J. Virology, 91: 22 e01316-17 (The first two authors contributed equally). JVI spotlight. 6. Li, D., Wang, X., von Schaewen, M., Tao, W., Zhang, Y., Heller, B., Hrebikova, G., Deng, Q., Sun, Q., ^#Ploss, A., ^#Zhong, J.,* ^#Huang, Z. (2017) Immunization with a subunit hepatitis C virus vaccine elicits pan-genotypic neutralizing antibodies and intra-hepatic T-cell responses in non-human primates. J. Infectious Diseases, 215(12):1824-1831 (^#corresponding author). 7. Cao, R., Xu, Y., Zhang, T., Yang, J., Yuan, Y., Hao, P., Shi, Y., ^#Zhong, J., ^#Zhong, W. (2017) Pediatric drug nitazoxanide: a potential choice for control of Zika. Open Forum Infectious Diseases, 4(1):ofx009 (The first two authors contributed equally; ^#corresponding author). 8. Tong, Y., Chi, X., Yang, W., Zhong, J.* (2017) Functional analysis of HCV envelope protein E1 using a trans-complementation system reveals a dual role of a putative fusion peptide of E1 in both HCV entry and morphogenesis. J. Virology, 91:7 16 e02468-16 9. Hei, L. and Zhong, J. (2017) LGP2 plays an essential role in HCV infection-induced interferon responses. Hepatology, 65(5):1478-1491. 10. Yan, Y., He, Y., Boson, B., Wang, X., Cosset, F.L., Zhong, J. (2017) A point mutation in the N-terminal amphiphathic helix α0 in NS3 promotes HCV assembly by altering core localization to ER and facilitating virus budding. J. Virology, 91(6). pii: e02399-16 (The first two authors contributed equally). 11. Tao, W., Gan T., Lu, J., Zhong, J.* (2017) A Profiling Study of a Newly Developed HCVcc Strain PR63cc’s Sensitivity to Direct-acting Antivirals. Antiviral Research, 139:18-24 (The first two authors contributed equally). 12. Li, D., von Schaewen, M., Wang, X., Tao, W., Zhang, Y., Li, L., Heller, B., Hrebikova, G., Deng, Q., ^#Ploss, A., ^#Zhong, J.,* ^#Huang, Z. (2016) Altered glycosylation patterns increase immunogenicity of a subunit HCV vaccine inducing neutralizing antibodies which confer protection in mice. J. Virology, 90:10486-10498 (^#corresponding author). 13. Xu, Y., and Zhong, J. (2016) Innate immunity against hepatitis C virus. Current Opinion in Immunology. 42:98-104. 14. Li, D., Huang, Z. and Zhong, J. (2015) Hepatitis C virus vaccine development: old challenges and new opportunities. National Science Review, 2 (3): 285. 15. Xiang, Y., Tang, J., Tao, W., Cao, X., Song, B. and Zhong, J. (2015) Identification of cholesterol-25-hydroxylase as a novel host restriction factor as a part of primary innate immune responses against hepatitis C virus infection. J. Virology, 89:6805–6816. 16. Cao, X., Ding, Q., Lu J., Tao, W., Huang, B., Zhao, Y., Niu, J., Liu, Y.J. and Zhong, J. (2015) MDA5 Plays a Critical Role in Interferon Response during Hepatitis C Virus Infection. J. Hepatology. 62:771–778 (The first two authors contributed equally) . 17. Chen, W., Xu, Y., Li, H., Tao, W., Xiang, Y., Huang, B., Niu, J., ^#Zhong, J. and ^#Meng, G. (2014) HCV Genomic RNA Activates the NLRP3 Inflammasome in Human Myeloid Cells. PLoS One,* 9(1):e84953 (The first two authors contributed equally; ^#corresponding author). 18. Lu, J., Xiang, Y., Tao, W., Li, Q., Wang, N., Gao, Y., Xiang, X., Xie, Q., and Zhong, J. (2014) A novel strategy to develop robust infectious hepatitis C virus cell culture system directly from a clinical isolate. J. Virology, 88, 1484-1491. 19. Xu, Y., Li, H., Chen, W., Yao, X., Xing, Y., Wang, X., ^#Zhong, J. and ^#Meng, G. (2013) Mycoplasma hyorhinis Activates the NLRP3 Inflammasome and Promotes Migration and Invasion of Gastric Cancer Cells. PLoS One,* 8(11): e77955 (The first two authors contributed equally; ^#corresponding author). 20. Qi, Y., Xiang, Y., Wang, J., Qi, Y., Li, J., ^#Niu, J., and ^#Zhong, J. (2013) Inhibition of Hepatitis C Virus Infection by Polyoxometalates. Antiviral Research, 100(2), 392-398 (The first two authors contributed equally; ^#corresponding author). 21. Lu, J., Tao, W., Li, R., Xiang, Y., Zhang, N., Xiang, X., ^#Xie, Q., and ^#Zhong, J. (2013) Construction and characterization of infectious hepatitis C virus chimera containing structural proteins directly from genotype 1b clinical isolates. Virology, 443(1), 80-88 (^#corresponding author). 22. Ding, Q., Cao, X., Lu J., Huang, B., Liu, Y.J., Kato, N., Shu, H., and Zhong, J. (2013) Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. J. Hepatology. 59(1):52-58 (The first two authors contributed equally). 23. Kang, X., Chen, X., He, Y., Guo, D., Guo, L., ^#Zhong, J., ^#Shu, H. (2013) DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus. Virology, 435(2), 385-394 (^#corresponding author). 24. Ding, Q., Huang, B., Lu, J., Liu, Y.J., and Zhong, J. (2012) Hepatitis C virus NS3/4A protease blocks IL-28 production. European J. Immunology, 42, 2374-82. 25. He, Y., Weng, L., Li, R., Li, L., Toyoda, T., and Zhong, J. (2012) The N-terminal helix ？₀ of hepatitis C virus NS3 protein dictates the subcellular localization and stability of NS3/NS4A complex. Virology, 422, 214-223. 26. Li, R., Qin, Y., He, Y., Tao, W., Zhang, N., Tsai, C., Zhou, P., Zhong, J. (2011) Production of hepatitis C virus lacking the envelope-encoding genes for single-cycle infection by providing homologous envelope proteins or vesicular stomatitis virus glycoproteins in trans. J.Virology,85, 2138-2147. 27. Xiang, X., Lu, J., Dong, Z., Zhou, H., Tao, W., Guo, Q., Zhou, X., ^#Xie, Q., ^#Zhong, J.* (2011) Viral Sequence Evolution in Chinese Genotype 1b Chronic Hepatitis C Patients Experiencing Unsuccessful Interferon Treatment. Infect. Genet. Evol., 11, 382-390 (^#corresponding author). 28. Tao, W., Xu, C., Ding, Q., Li, R., Xiang, Y., Chung, J., and Zhong, J. (2009) A Single Point Mutation in E2 Enhances Hepatitis C Virus Infectivity and Alters Lipoprotein Association of Viral Particles. Virology, 395, 67–76.

Research progress:

1. ^#Tong, Y., Lavillette, D., Li, Q. and ^#Zhong, J. (2018) Role of hepatitis c virus envelope glycoprotein E1 in virus entry and assembly. Front. Immunol. 9:1411. doi: 10.3389/fimmu.2018.01411 (^#corresponding author).

2. *Liang, Y., *Cao, X., Ding, Q., Zhao, Y., He, Z., Zhong, J. (2018) Hepatitis C virus NS4B induces the degradation of TRIF to inhibit TLR3-mediated interferon signaling pathway. PLOS Pathogens. 14(5):e1007075 (*The first two authors contributed equally).

3. Li, Q., Tong Y., Xu, Y., ^#Niu, J., ^#Zhong, J. (2018) Genetic Analysis of Serum-derived Defective Hepatitis C Virus Genomes Revealed Novel Viral Cis-Elements for Virus Replication and Assembly. J. Virology, 92(7). pii: e02182-17 (^#corresponding author).

4. *Wang, X., *Yan, Y., Gan, T., Yang, X., Li, D., Zhou, D., Sun, Q., ^#Huang, Z., ^#Zhong, J. (2017) A trivalent HCV vaccine elicits broad and synergistic polyclonal antibody response in mice and rhesus monkey. Gut, doi: 10.1136/gutjnl-2017-314870. (*The first two authors contributed equally;^#corresponding author).

5. *Tao, W., *Gan T., Guo, M., Xu, Y., Zhong, J. (2017) Novel Stable Ebola Virus Minigenome Replicon Reveals Remarkable Stability of the Viral Genome. J. Virology, 91: 22 e01316-17 (*The first two authors contributed equally). JVI spotlight.

6. Li, D., Wang, X., von Schaewen, M., Tao, W., Zhang, Y., Heller, B., Hrebikova, G., Deng, Q., Sun, Q., ^#Ploss, A., ^#Zhong, J., ^#Huang, Z. (2017) Immunization with a subunit hepatitis C virus vaccine elicits pan-genotypic neutralizing antibodies and intra-hepatic T-cell responses in non-human primates. J. Infectious Diseases, 215(12):1824-1831 (^#corresponding author).

7. *Cao, R., *Xu, Y., Zhang, T., Yang, J., Yuan, Y., Hao, P., Shi, Y., ^#Zhong, J., ^#Zhong, W. (2017) Pediatric drug nitazoxanide: a potential choice for control of Zika. Open Forum Infectious Diseases, 4(1):ofx009 (*The first two authors contributed equally; ^#corresponding author).

8. Tong, Y., Chi, X., Yang, W., Zhong, J. (2017) Functional analysis of HCV envelope protein E1 using a trans-complementation system reveals a dual role of a putative fusion peptide of E1 in both HCV entry and morphogenesis. J. Virology, 91:7 16 e02468-16

9. Hei, L. and Zhong, J. (2017) LGP2 plays an essential role in HCV infection-induced interferon responses. Hepatology, 65(5):1478-1491.

10. *Yan, Y., *He, Y., Boson, B., Wang, X., Cosset, F.L., Zhong, J. (2017) A point mutation in the N-terminal amphiphathic helix α0 in NS3 promotes HCV assembly by altering core localization to ER and facilitating virus budding. J. Virology, 91(6). pii: e02399-16 (*The first two authors contributed equally).

11. *Tao, W., *Gan T., Lu, J., Zhong, J. (2017) A Profiling Study of a Newly Developed HCVcc Strain PR63cc’s Sensitivity to Direct-acting Antivirals. Antiviral Research, 139:18-24 (*The first two authors contributed equally).

12. Li, D., von Schaewen, M., Wang, X., Tao, W., Zhang, Y., Li, L., Heller, B., Hrebikova, G., Deng, Q., ^#Ploss, A., ^#Zhong, J., ^#Huang, Z. (2016) Altered glycosylation patterns increase immunogenicity of a subunit HCV vaccine inducing neutralizing antibodies which confer protection in mice. J. Virology, 90:10486-10498 (^#corresponding author).

13. Xu, Y., and Zhong, J. (2016) Innate immunity against hepatitis C virus. Current Opinion in Immunology. 42:98-104.

14. Li, D., Huang, Z. and Zhong, J. (2015) Hepatitis C virus vaccine development: old challenges and new opportunities. National Science Review, 2 (3): 285.

15. Xiang, Y., Tang, J., Tao, W., Cao, X., Song, B. and Zhong, J. (2015) Identification of cholesterol-25-hydroxylase as a novel host restriction factor as a part of primary innate immune responses against hepatitis C virus infection. J. Virology, 89:6805–6816.

16. *Cao, X., *Ding, Q., Lu J., Tao, W., Huang, B., Zhao, Y., Niu, J., Liu, Y.J. and Zhong, J. (2015) MDA5 Plays a Critical Role in Interferon Response during Hepatitis C Virus Infection. J. Hepatology. 62:771–778 (*The first two authors contributed equally) .

17. *Chen, W., *Xu, Y., Li, H., Tao, W., Xiang, Y., Huang, B., Niu, J., ^#Zhong, J. and ^#Meng, G. (2014) HCV Genomic RNA Activates the NLRP3 Inflammasome in Human Myeloid Cells. PLoS One, 9(1):e84953 (*The first two authors contributed equally; ^#corresponding author).

18. Lu, J., Xiang, Y., Tao, W., Li, Q., Wang, N., Gao, Y., Xiang, X., Xie, Q., and Zhong, J. (2014) A novel strategy to develop robust infectious hepatitis C virus cell culture system directly from a clinical isolate. J. Virology, 88, 1484-1491.

19. *Xu, Y., *Li, H., Chen, W., Yao, X., Xing, Y., Wang, X., ^#Zhong, J. and ^#Meng, G. (2013) Mycoplasma hyorhinis Activates the NLRP3 Inflammasome and Promotes Migration and Invasion of Gastric Cancer Cells. PLoS One, 8(11): e77955 (*The first two authors contributed equally; ^#corresponding author).

20. *Qi, Y., *Xiang, Y., Wang, J., Qi, Y., Li, J., ^#Niu, J., and ^#Zhong, J. (2013) Inhibition of Hepatitis C Virus Infection by Polyoxometalates. Antiviral Research, 100(2), 392-398 (*The first two authors contributed equally; ^#corresponding author).

21. Lu, J., Tao, W., Li, R., Xiang, Y., Zhang, N., Xiang, X., ^#Xie, Q., and ^#Zhong, J. (2013) Construction and characterization of infectious hepatitis C virus chimera containing structural proteins directly from genotype 1b clinical isolates. Virology, 443(1), 80-88 (^#corresponding author).

22. *Ding, Q., *Cao, X., Lu J., Huang, B., Liu, Y.J., Kato, N., Shu, H., and Zhong, J. (2013) Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. J. Hepatology. 59(1):52-58 (*The first two authors contributed equally).

23. Kang, X., Chen, X., He, Y., Guo, D., Guo, L., ^#Zhong, J., ^#Shu, H. (2013) DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus. Virology, 435(2), 385-394 (^#corresponding author).

24. Ding, Q., Huang, B., Lu, J., Liu, Y.J., and Zhong, J. (2012) Hepatitis C virus NS3/4A protease blocks IL-28 production. European J. Immunology, 42, 2374-82.

25. He, Y., Weng, L., Li, R., Li, L., Toyoda, T., and Zhong, J. (2012) The N-terminal helix ？₀ of hepatitis C virus NS3 protein dictates the subcellular localization and stability of NS3/NS4A complex. Virology, 422, 214-223.

26. Li, R., Qin, Y., He, Y., Tao, W., Zhang, N., Tsai, C., Zhou, P., Zhong, J. (2011) Production of hepatitis C virus lacking the envelope-encoding genes for single-cycle infection by providing homologous envelope proteins or vesicular stomatitis virus glycoproteins in trans. J.Virology,85, 2138-2147.

27. Xiang, X., Lu, J., Dong, Z., Zhou, H., Tao, W., Guo, Q., Zhou, X., ^#Xie, Q., ^#Zhong, J. (2011) Viral Sequence Evolution in Chinese Genotype 1b Chronic Hepatitis C Patients Experiencing Unsuccessful Interferon Treatment. Infect. Genet. Evol., 11, 382-390 (^#corresponding author).

28. Tao, W., Xu, C., Ding, Q., Li, R., Xiang, Y., Chung, J., and Zhong, J. (2009) A Single Point Mutation in E2 Enhances Hepatitis C Virus Infectivity and Alters Lipoprotein Association of Viral Particles. Virology, 395, 67–76.

Laboratory members:
Research Associate Professor: Yongfen Xu Ph.D. , Yimin Tong Ph.D. Research Secretary: Jiameng Xia Graduate Students: PhD students (University of Chinese Academy of Sciences): Zhenliang He, Tianyu Gan, Qiankun Yang, STEVE LEUMI YATCHOUKEU M.S. students (University of Chinese Academy of Sciences): Xiaoyou Hu, Yifan Xing, Sichao Ye Ph.D. students (ShanghaiTech University): Mingzhe Guo, Yisha Liang, Lin Han, Peilan Lou M.S. students (ShanghaiTech University): Fangling Tian, Ziyue Ma Post-doctoral fellows: Yongfen Xu, Yimin Tong, Wanyin Tao, Fahed Parvaiz Ph.D. students: Wanyin Tao, Ying He, Rui Li, Qiang Ding, Jie Lu, Xuezhi Cao, Yu Xiang, Lei He, Tao Yu, Yu Yan, Qingchao Li, Xuesong Wang, Yanan Zhao M.S. students: Liqing Ye, Lei Xie Co-supervised Ph.D. students: Xiaogang Xiang, Dapeng Li Ex-members:

Laboratory members:

Research Associate Professor: Yongfen Xu Ph.D. , Yimin Tong Ph.D.

Research Secretary: Jiameng Xia

Graduate Students:

PhD students (University of Chinese Academy of Sciences): Zhenliang He, Tianyu Gan, Qiankun Yang, STEVE LEUMI YATCHOUKEU

M.S. students (University of Chinese Academy of Sciences): Xiaoyou Hu, Yifan Xing, Sichao Ye

Ph.D. students (ShanghaiTech University): Mingzhe Guo, Yisha Liang, Lin Han, Peilan Lou

M.S. students (ShanghaiTech University): Fangling Tian, Ziyue Ma

Post-doctoral fellows: Yongfen Xu, Yimin Tong, Wanyin Tao, Fahed Parvaiz

Ph.D. students: Wanyin Tao, Ying He, Rui Li, Qiang Ding, Jie Lu, Xuezhi Cao, Yu Xiang, Lei He, Tao Yu, Yu Yan, Qingchao Li, Xuesong Wang, Yanan Zhao

M.S. students: Liqing Ye, Lei Xie

Co-supervised Ph.D. students: Xiaogang Xiang, Dapeng Li

Ex-members: