专家人才库数据----中国科学院上海巴斯德研究所

博士生导师

姓名:	钟劲	性别:	男
学历:	博士
专家类别:	中国科学院特聘研究员	职称:	研究员
所属部门:	病毒性肝炎研究组	学科类别:	病毒学
联系电话:	021-54923143	电子邮箱:	jzhong@sibs.ac.cn
通讯地址:	上海市徐汇区岳阳路320号

个人简介：
钟劲，研究员，博士生导师。1994年获四川大学学士学位；1997年获北京大学硕士学位；2003年获美国德克萨斯大学博士学位，随后到美国Scripps研究所做博士后，一直从事HCV的研究，期间建立了世界上第一个HCV的细胞感染模型。2007年受聘中国科学院上海巴斯德研究所研究员。现担任病毒性肝炎研究组研究组长，博士生导师。

个人简介：

　　钟劲，研究员，博士生导师。1994年获四川大学学士学位；1997年获北京大学硕士学位；2003年获美国德克萨斯大学博士学位，随后到美国Scripps研究所做博士后，一直从事HCV的研究，期间建立了世界上第一个HCV的细胞感染模型。2007年受聘中国科学院上海巴斯德研究所研究员。现担任病毒性肝炎研究组研究组长，博士生导师。

研究方向：
本实验室研究重要RNA病毒病原体的分子病毒学、细胞生物学、固有免疫应答以及抗病毒疫苗和药物的研发，主要研究对象包括丙型肝炎病毒（HCV）、埃博拉病毒（EBOV）、寨卡病毒（ZIKV）等。HCV能导致急性和慢性肝炎以及肝癌。近年来慢性丙肝的治疗研究有了很大的进展，但是由于缺乏丙肝疫苗，丙肝问题仍然威胁着人类的健康。HCV作为一个正链RNA模式病毒，遗传多样性高。其病毒颗粒与宿主来源的脂蛋白复合物高度关联，在其生命周期和宿主相互作用上具有鲜明的特点。EBOV和ZIKV是新发突发致病性RNA病毒，近年来多次引起全球或区域流行，给世界公共卫生健康造成较大的威胁。我们在分子及细胞水平上研究这些RNA病毒感染的生物学机制，阐明病毒学、免疫学和细胞生物学中重要的科学问题，找到更好的途径和手段来预防和治疗病毒感染。

研究方向：

　　本实验室研究重要RNA病毒病原体的分子病毒学、细胞生物学、固有免疫应答以及抗病毒疫苗和药物的研发，主要研究对象包括丙型肝炎病毒（HCV）、埃博拉病毒（EBOV）、寨卡病毒（ZIKV）等。HCV能导致急性和慢性肝炎以及肝癌。近年来慢性丙肝的治疗研究有了很大的进展，但是由于缺乏丙肝疫苗，丙肝问题仍然威胁着人类的健康。HCV作为一个正链RNA模式病毒，遗传多样性高。其病毒颗粒与宿主来源的脂蛋白复合物高度关联，在其生命周期和宿主相互作用上具有鲜明的特点。EBOV和ZIKV是新发突发致病性RNA病毒，近年来多次引起全球或区域流行，给世界公共卫生健康造成较大的威胁。我们在分子及细胞水平上研究这些RNA病毒感染的生物学机制，阐明病毒学、免疫学和细胞生物学中重要的科学问题，找到更好的途径和手段来预防和治疗病毒感染。

科研进展：
部分近期通讯作者论文 1. Guo, M., Lu, J., Gan, T., Xiang. X., Xu, Y., Xie, Q. and Zhong, J. (2019) Construction and characterization of Genotype-3 hepatitis C virus replicon revealed critical genotype-3-specific polymorphism for drug resistance and viral fitness. Antiviral Research, 171, 104612. 2. Yan, Y., Wang, X., Lou, P., Hu, Z., Qu, P., Li, D., Li, Q., Xu, Y., Niu, J., He, Y., #Zhong, J., Huang, Z. (2019) A nanoparticle-based HCV vaccine with enhanced potency. J. Infectious Diseases, pii: jiz228. doi: 10.1093/infdis/jiz228. [Epub ahead of print] (*The first two authors contributed equally; # co-corresponding author). 3. Zhao, Y., Cao, X., Guo, M., Wang, X., Yu, T., Ye. L., Han, L., Hei, L., Tao. W., Tong Y., Xu, Y. and Zhong, J. (2018) Neuralized E3 Ubiquitin Protein Ligase 3 is an inducible antiviral effector to inhibit HCV assembly by targeting viral E1 glycoprotein. J. Virology, 92(21). pii: e01123-18. JVI spotlight. 4. ^#Tong, Y., Lavillette, D., Li, Q. and ^#Zhong, J. (2018) Role of hepatitis c virus envelope glycoprotein E1 in virus entry and assembly. Front. Immunol. 9:1411. doi: 10.3389/fimmu.2018.01411 (^#corresponding author). 5. Liang, Y., Cao, X., Ding, Q., Zhao, Y., He, Z., Zhong, J. (2018) Hepatitis C virus NS4B induces the degradation of TRIF to inhibit TLR3-mediated interferon signaling pathway. PLOS Pathogens. 14(5):e1007075 (The first two authors contributed equally). 6. Li, Q., Tong Y., Xu, Y., #Niu, J., #Zhong, J.* (2018) Genetic Analysis of Serum-derived Defective Hepatitis C Virus Genomes Revealed Novel Viral Cis-Elements for Virus Replication and Assembly. J. Virology, 92(7). pii: e02182-17 (#corresponding author). 7. Wang, X., Yan, Y., Gan, T., Yang, X., Li, D., Zhou, D., Sun, Q., #Huang, Z., #Zhong, J. (2017) A trivalent HCV vaccine elicits broad and synergistic polyclonal antibody response in mice and rhesus monkey. Gut, doi: 10.1136/gutjnl-2017-314870. (The first two authors contributed equally; #corresponding author). 8. Tao, W., Gan T., Guo, M., Xu, Y., Zhong, J.* (2017) Novel Stable Ebola Virus Minigenome Replicon Reveals Remarkable Stability of the Viral Genome. J. Virology, 91: 22 e01316-17 (The first two authors contributed equally). JVI spotlight. 9. Li, D., Wang, X., von Schaewen, M., Tao, W., Zhang, Y., Heller, B., Hrebikova, G., Deng, Q., Sun, Q., #Ploss, A., #Zhong, J., #Huang, Z. (2017) Immunization with a subunit hepatitis C virus vaccine elicits pan-genotypic neutralizing antibodies and intra-hepatic T-cell responses in non-human primates. J. Infectious Diseases, 215(12):1824-1831 (#corresponding author). 10. Cao, R., Xu, Y., Zhang, T., Yang, J., Yuan, Y., Hao, P., Shi, Y., #Zhong, J., #Zhong, W. (2017) Pediatric drug nitazoxanide: a potential choice for control of Zika. Open Forum Infectious Diseases, 4(1):ofx009 (The first two authors contributed equally; #corresponding author). 11. Tong, Y., Chi, X., Yang, W., Zhong, J. (2017) Functional analysis of HCV envelope protein E1 using a trans-complementation system reveals a dual role of a putative fusion peptide of E1 in both HCV entry and morphogenesis. J. Virology, 91:7 16 e02468-16 12. Hei, L. and Zhong, J. (2017) LGP2 plays an essential role in HCV infection-induced interferon responses. Hepatology, 65(5):1478-1491. 13. Yan, Y., He, Y., Boson, B., Wang, X., Cosset, F.L., Zhong, J. (2017) A point mutation in the N-terminal amphiphathic helix α0 in NS3 promotes HCV assembly by altering core localization to ER and facilitating virus budding. J. Virology, 91(6). pii: e02399-16 (The first two authors contributed equally). 14. Tao, W., Gan T., Lu, J., Zhong, J.* (2017) A Profiling Study of a Newly Developed HCVcc Strain PR63cc’s Sensitivity to Direct-acting Antivirals. Antiviral Research, 139:18-24 (The first two authors contributed equally). 15. Li, D., von Schaewen, M., Wang, X., Tao, W., Zhang, Y., Li, L., Heller, B., Hrebikova, G., Deng, Q., #Ploss, A., #Zhong, J., #Huang, Z. (2016) Altered glycosylation patterns increase immunogenicity of a subunit HCV vaccine inducing neutralizing antibodies which confer protection in mice. J. Virology, 90:10486-10498 (#corresponding author). 16. Xu, Y., and Zhong, J.* (2016) Innate immunity against hepatitis C virus. Current Opinion in Immunology. 42:98-104. 17. Li, D., Huang, Z. and Zhong, J. (2015) Hepatitis C virus vaccine development: old challenges and new opportunities. National Science Review, 2 (3): 285. 18. Xiang, Y., Tang, J., Tao, W., Cao, X., Song, B. and Zhong, J. (2015) Identification of cholesterol-25-hydroxylase as a novel host restriction factor as a part of primary innate immune responses against hepatitis C virus infection. J. Virology, 89:6805–6816. 19. Cao, X., Ding, Q., Lu J., Tao, W., Huang, B., Zhao, Y., Niu, J., Liu, Y.J. and Zhong, J. (2015) MDA5 Plays a Critical Role in Interferon Response during Hepatitis C Virus Infection. J. Hepatology. 62:771–778 (The first two authors contributed equally) . 20. Chen, W., Xu, Y., Li, H., Tao, W., Xiang, Y., Huang, B., Niu, J., #Zhong, J.* and #Meng, G. (2014) HCV Genomic RNA Activates the NLRP3 Inflammasome in Human Myeloid Cells. PLoS One, 9(1):e84953 (The first two authors contributed equally; #corresponding author). 21. Lu, J., Xiang, Y., Tao, W., Li, Q., Wang, N., Gao, Y., Xiang, X., Xie, Q., and Zhong, J.* (2014) A novel strategy to develop robust infectious hepatitis C virus cell culture system directly from a clinical isolate. J. Virology, 88, 1484-1491. 22. Xu, Y., Li, H., Chen, W., Yao, X., Xing, Y., Wang, X., #Zhong, J. and #Meng, G. (2013) Mycoplasma hyorhinis Activates the NLRP3 Inflammasome and Promotes Migration and Invasion of Gastric Cancer Cells. PLoS One, 8(11): e77955 (The first two authors contributed equally; #corresponding author). 23. Qi, Y., Xiang, Y., Wang, J., Qi, Y., Li, J., #Niu, J., and #Zhong, J.* (2013) Inhibition of Hepatitis C Virus Infection by Polyoxometalates. Antiviral Research, 100(2), 392-398 (The first two authors contributed equally; #corresponding author). 24. Lu, J., Tao, W., Li, R., Xiang, Y., Zhang, N., Xiang, X., #Xie, Q., and #Zhong, J.* (2013) Construction and characterization of infectious hepatitis C virus chimera containing structural proteins directly from genotype 1b clinical isolates. Virology, 443(1), 80-88 (#corresponding author). 25. Ding, Q., Cao, X., Lu J., Huang, B., Liu, Y.J., Kato, N., Shu, H., and Zhong, J. (2013) Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. J. Hepatology. 59(1):52-58 (The first two authors contributed equally). 26. Kang, X., Chen, X., He, Y., Guo, D., Guo, L., #Zhong, J., #Shu, H. (2013) DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus. Virology, 435(2), 385-394 (#corresponding author). 27. Ding, Q., Huang, B., Lu, J., Liu, Y.J., and Zhong, J.* (2012) Hepatitis C virus NS3/4A protease blocks IL-28 production. European J. Immunology, 42, 2374-82. 28. He, Y., Weng, L., Li, R., Li, L., Toyoda, T., and Zhong, J. (2012) The N-terminal helix a0 of hepatitis C virus NS3 protein dictates the subcellular localization and stability of NS3/NS4A complex. Virology, 422, 214-223. 29. Li, R., Qin, Y., He, Y., Tao, W., Zhang, N., Tsai, C., Zhou, P., Zhong, J. (2011) Production of hepatitis C virus lacking the envelope-encoding genes for single-cycle infection by providing homologous envelope proteins or vesicular stomatitis virus glycoproteins in trans. J.Virology,85, 2138-2147. 30. Xiang, X., Lu, J., Dong, Z., Zhou, H., Tao, W., Guo, Q., Zhou, X., #Xie, Q., #Zhong, J. (2011) Viral Sequence Evolution in Chinese Genotype 1b Chronic Hepatitis C Patients Experiencing Unsuccessful Interferon Treatment. Infect. Genet. Evol., 11, 382-390 (#corresponding author). 31. Tao, W., Xu, C., Ding, Q., Li, R., Xiang, Y., Chung, J., and Zhong, J. (2009) A Single Point Mutation in E2 Enhances Hepatitis C Virus Infectivity and Alters Lipoprotein Association of Viral Particles. Virology, 395, 67–76.

科研进展：

部分近期通讯作者论文

1. Guo, M., Lu, J., Gan, T., Xiang. X., Xu, Y., Xie, Q. and Zhong, J. (2019) Construction and characterization of Genotype-3 hepatitis C virus replicon revealed critical genotype-3-specific polymorphism for drug resistance and viral fitness. Antiviral Research, 171, 104612.

2. *Yan, Y., *Wang, X., Lou, P., Hu, Z., Qu, P., Li, D., Li, Q., Xu, Y., Niu, J., He, Y., #Zhong, J., Huang, Z. (2019) A nanoparticle-based HCV vaccine with enhanced potency. J. Infectious Diseases, pii: jiz228. doi: 10.1093/infdis/jiz228. [Epub ahead of print] (*The first two authors contributed equally; # co-corresponding author).

3. Zhao, Y., Cao, X., Guo, M., Wang, X., Yu, T., Ye. L., Han, L., Hei, L., Tao. W., Tong Y., Xu, Y. and Zhong, J. (2018) Neuralized E3 Ubiquitin Protein Ligase 3 is an inducible antiviral effector to inhibit HCV assembly by targeting viral E1 glycoprotein. J. Virology, 92(21). pii: e01123-18. JVI spotlight.

4. ^#Tong, Y., Lavillette, D., Li, Q. and ^#Zhong, J. (2018) Role of hepatitis c virus envelope glycoprotein E1 in virus entry and assembly. Front. Immunol. 9:1411. doi: 10.3389/fimmu.2018.01411 (^#corresponding author).

5. *Liang, Y., *Cao, X., Ding, Q., Zhao, Y., He, Z., Zhong, J. (2018) Hepatitis C virus NS4B induces the degradation of TRIF to inhibit TLR3-mediated interferon signaling pathway. PLOS Pathogens. 14(5):e1007075 (*The first two authors contributed equally).

6. Li, Q., Tong Y., Xu, Y., #Niu, J., #Zhong, J. (2018) Genetic Analysis of Serum-derived Defective Hepatitis C Virus Genomes Revealed Novel Viral Cis-Elements for Virus Replication and Assembly. J. Virology, 92(7). pii: e02182-17 (#corresponding author).

7. *Wang, X., *Yan, Y., Gan, T., Yang, X., Li, D., Zhou, D., Sun, Q., #Huang, Z., #Zhong, J. (2017) A trivalent HCV vaccine elicits broad and synergistic polyclonal antibody response in mice and rhesus monkey. Gut, doi: 10.1136/gutjnl-2017-314870. (*The first two authors contributed equally; #corresponding author).

8. *Tao, W., *Gan T., Guo, M., Xu, Y., Zhong, J. (2017) Novel Stable Ebola Virus Minigenome Replicon Reveals Remarkable Stability of the Viral Genome. J. Virology, 91: 22 e01316-17 (*The first two authors contributed equally). JVI spotlight.

9. Li, D., Wang, X., von Schaewen, M., Tao, W., Zhang, Y., Heller, B., Hrebikova, G., Deng, Q., Sun, Q., #Ploss, A., #Zhong, J., #Huang, Z. (2017) Immunization with a subunit hepatitis C virus vaccine elicits pan-genotypic neutralizing antibodies and intra-hepatic T-cell responses in non-human primates. J. Infectious Diseases, 215(12):1824-1831 (#corresponding author).

10. *Cao, R., *Xu, Y., Zhang, T., Yang, J., Yuan, Y., Hao, P., Shi, Y., #Zhong, J., #Zhong, W. (2017) Pediatric drug nitazoxanide: a potential choice for control of Zika. Open Forum Infectious Diseases, 4(1):ofx009 (*The first two authors contributed equally; #corresponding author).

11. Tong, Y., Chi, X., Yang, W., Zhong, J. (2017) Functional analysis of HCV envelope protein E1 using a trans-complementation system reveals a dual role of a putative fusion peptide of E1 in both HCV entry and morphogenesis. J. Virology, 91:7 16 e02468-16

12. Hei, L. and Zhong, J. (2017) LGP2 plays an essential role in HCV infection-induced interferon responses. Hepatology, 65(5):1478-1491.

13. *Yan, Y., *He, Y., Boson, B., Wang, X., Cosset, F.L., Zhong, J. (2017) A point mutation in the N-terminal amphiphathic helix α0 in NS3 promotes HCV assembly by altering core localization to ER and facilitating virus budding. J. Virology, 91(6). pii: e02399-16 (*The first two authors contributed equally).

14. *Tao, W., *Gan T., Lu, J., Zhong, J. (2017) A Profiling Study of a Newly Developed HCVcc Strain PR63cc’s Sensitivity to Direct-acting Antivirals. Antiviral Research, 139:18-24 (*The first two authors contributed equally).

15. Li, D., von Schaewen, M., Wang, X., Tao, W., Zhang, Y., Li, L., Heller, B., Hrebikova, G., Deng, Q., #Ploss, A., #Zhong, J., #Huang, Z. (2016) Altered glycosylation patterns increase immunogenicity of a subunit HCV vaccine inducing neutralizing antibodies which confer protection in mice. J. Virology, 90:10486-10498 (#corresponding author).

16. Xu, Y., and Zhong, J. (2016) Innate immunity against hepatitis C virus. Current Opinion in Immunology. 42:98-104.

17. Li, D., Huang, Z. and Zhong, J. (2015) Hepatitis C virus vaccine development: old challenges and new opportunities. National Science Review, 2 (3): 285.

18. Xiang, Y., Tang, J., Tao, W., Cao, X., Song, B. and Zhong, J. (2015) Identification of cholesterol-25-hydroxylase as a novel host restriction factor as a part of primary innate immune responses against hepatitis C virus infection. J. Virology, 89:6805–6816.

19. *Cao, X., *Ding, Q., Lu J., Tao, W., Huang, B., Zhao, Y., Niu, J., Liu, Y.J. and Zhong, J. (2015) MDA5 Plays a Critical Role in Interferon Response during Hepatitis C Virus Infection. J. Hepatology. 62:771–778 (*The first two authors contributed equally) .

20. *Chen, W., *Xu, Y., Li, H., Tao, W., Xiang, Y., Huang, B., Niu, J., #Zhong, J. and #Meng, G. (2014) HCV Genomic RNA Activates the NLRP3 Inflammasome in Human Myeloid Cells. PLoS One, 9(1):e84953 (*The first two authors contributed equally; #corresponding author).

21. Lu, J., Xiang, Y., Tao, W., Li, Q., Wang, N., Gao, Y., Xiang, X., Xie, Q., and Zhong, J. (2014) A novel strategy to develop robust infectious hepatitis C virus cell culture system directly from a clinical isolate. J. Virology, 88, 1484-1491.

22. *Xu, Y., *Li, H., Chen, W., Yao, X., Xing, Y., Wang, X., #Zhong, J. and #Meng, G. (2013) Mycoplasma hyorhinis Activates the NLRP3 Inflammasome and Promotes Migration and Invasion of Gastric Cancer Cells. PLoS One, 8(11): e77955 (*The first two authors contributed equally; #corresponding author).

23. *Qi, Y., *Xiang, Y., Wang, J., Qi, Y., Li, J., #Niu, J., and #Zhong, J. (2013) Inhibition of Hepatitis C Virus Infection by Polyoxometalates. Antiviral Research, 100(2), 392-398 (*The first two authors contributed equally; #corresponding author).

24. Lu, J., Tao, W., Li, R., Xiang, Y., Zhang, N., Xiang, X., #Xie, Q., and #Zhong, J. (2013) Construction and characterization of infectious hepatitis C virus chimera containing structural proteins directly from genotype 1b clinical isolates. Virology, 443(1), 80-88 (#corresponding author).

25. *Ding, Q., *Cao, X., Lu J., Huang, B., Liu, Y.J., Kato, N., Shu, H., and Zhong, J. (2013) Hepatitis C virus NS4B blocks the interaction of STING and TBK1 to evade host innate immunity. J. Hepatology. 59(1):52-58 (*The first two authors contributed equally).

26. Kang, X., Chen, X., He, Y., Guo, D., Guo, L., #Zhong, J., #Shu, H. (2013) DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus. Virology, 435(2), 385-394 (#corresponding author).

27. Ding, Q., Huang, B., Lu, J., Liu, Y.J., and Zhong, J. (2012) Hepatitis C virus NS3/4A protease blocks IL-28 production. European J. Immunology, 42, 2374-82.

28. He, Y., Weng, L., Li, R., Li, L., Toyoda, T., and Zhong, J. (2012) The N-terminal helix a0 of hepatitis C virus NS3 protein dictates the subcellular localization and stability of NS3/NS4A complex. Virology, 422, 214-223.

29. Li, R., Qin, Y., He, Y., Tao, W., Zhang, N., Tsai, C., Zhou, P., Zhong, J. (2011) Production of hepatitis C virus lacking the envelope-encoding genes for single-cycle infection by providing homologous envelope proteins or vesicular stomatitis virus glycoproteins in trans. J.Virology,85, 2138-2147.

30. Xiang, X., Lu, J., Dong, Z., Zhou, H., Tao, W., Guo, Q., Zhou, X., #Xie, Q., #Zhong, J. (2011) Viral Sequence Evolution in Chinese Genotype 1b Chronic Hepatitis C Patients Experiencing Unsuccessful Interferon Treatment. Infect. Genet. Evol., 11, 382-390 (#corresponding author).

31. Tao, W., Xu, C., Ding, Q., Li, R., Xiang, Y., Chung, J., and Zhong, J. (2009) A Single Point Mutation in E2 Enhances Hepatitis C Virus Infectivity and Alters Lipoprotein Association of Viral Particles. Virology, 395, 67–76.

实验室成员：
组长：钟劲博士，研究员其他成员：副研究员：徐咏芬博士、童一民博士科研秘书：夏佳梦研究生：国科大博士研究生：甘天喻、杨乾坤、STEVE LEUMI YATCHOUKEU、邢一帆、叶思超国科大硕士研究生：胡逍悠上科大博士研究生：梁轶莎、韩林、娄佩兰上科大硕士研究生：田芳玲、马子越已出站博士后：徐咏芬、童一民、陶万银已毕业博士研究生：陶万银、何莹、李瑞、丁强、卢捷、曹学智、向禹、黑蕾、于涛、颜雨、李庆超、王雪松、赵亚楠、何振亮、郭明哲已毕业硕士研究生：叶丽清、谢磊已毕业联合培养博士研究生：项晓刚、李大鹏

实验室成员：

组长：钟劲 博士，研究员

其他成员：

副研究员：徐咏芬博士、童一民博士

科研秘书：夏佳梦

研究生：

国科大博士研究生：甘天喻、杨乾坤、STEVE LEUMI YATCHOUKEU、邢一帆、叶思超

国科大硕士研究生：胡逍悠

上科大博士研究生：梁轶莎、韩林、娄佩兰

上科大硕士研究生：田芳玲、马子越

已出站博士后：徐咏芬、童一民、陶万银

已毕业博士研究生：陶万银、何莹、李瑞、丁强、卢捷、曹学智、向禹、黑蕾、于涛、颜雨、李庆超、王雪松、赵亚楠、何振亮、郭明哲

已毕业硕士研究生：叶丽清、谢磊

已毕业联合培养博士研究生：项晓刚、李大鹏